"Improved antioxidant status and lipid and protein peroxidation"

Lipid peroxidation, protein oxidant and antioxidant status
of muscle, intestine and hepatopancreas for juvenile Jian
carp (Cyprinus carpio var. Jian) fed graded levels of myo-inositol
Wei-Dan Jianga, b, Lin Fenga, b, Yang Liua, b, Jun Jianga, b,
Kai Hua, b, Shu-Hong Lia and Xiao-Qiu Zhoua, b, ,
aAnimal Nutrition Institute, Sichuan Agricultural University,
Sichuan, Ya’an, 625014, China
bKey Laboratory for Animal Disease-Resistance Nutrition of
China Ministry of Education, Sichuan Agricultural University,
Sichuan, Ya’an, 625014, China
Received 27 June 2009;  revised 14 August 2009;  accepted 21 October
2009.
Available online 29 October 2009.

Abstract
Lipid peroxidation, protein oxidant and antioxidant status of
muscle, intestine and hepatopancreas in juvenile Jian carp
(Cyprinus carpio var. Jian) fed graded levels of myo-inositol
(MI) (163.5, 232.7, 384.2, 535.8, 687.3, 838.8 and 990.3 mg/kg
diet) for 60 days were investigated.
Total tissue malondialdehyde (MDA) and protein carbonyl (PC)
content showed a downward trend to a point (P < 0.05).
Conversely, total tissue anti-hydroxyl radical (AHR), catalase
(CAT), glutathione-S-transferase (GST), glutathione peroxidase
(GPx), glutathione reducase (GR) activities and glutathione
(GSH) content were generally higher in MI-supplemented diets
than MI-unsupplemented diet (P < 0.05).
Muscle and intestinal superoxide dismutase (SOD), and
intestinal anti-superoxide anion (ASA) were increased by MI
supplementation (P < 0.05), whereas these parameters in the
other tissue showed no alterations (P > 0.05).
These results indicated that antioxidant status was improved,
and lipid peroxidation and protein oxidant were depressed in
muscle, intestine and hepatopancreas by MI.

Keywords: Jian carp (Cyprinus carpio var. Jian); Myo-inositol;
Lipid peroxidation; Protein oxidant; Hydroxyl radicals;
Antioxidant enzyme
doi:10.1016/j.foodchem.2009.10.062

Copyright © 2009 Published by Elsevier Ltd.

-----------

Phytate (Myo-inositol hexakisphosphate) inhibits cardiovascular
calcifications in rats.
Front Biosci. 2006 Jan 1;11:136-42.
Grases F, Sanchis P, Perello J, Isern B, Prieto RM, Fernandez-
Palomeque
C, Fiol M, Bonnin O, Torres JJ.
University Institute of Health Sciences Research (IUNICS), University
of Balearic Islands.

Calcification is an undesirable disorder, which frequently occurs in
the heart vessels. In general, the formation of calcific vascular
lesions involves complex physicochemical and molecular events.
Calcification (hydroxyapatite) is initiated by injury and is
progressed
by promoter factors and/or the deficit of inhibitory signals.
Myo-inositol hexakisphosphate (phytate, InsP6) is found in organs,
tissues and fluids of all mammals and exhibits an important capacity
as
a crystallization inhibitor of calcium salts in urine and soft
tissues.
The levels found clearly depend on the dietary intake but it can also
be absorbed topically. In this paper, the capacity of InsP6 as a
potential inhibitor of cardiovascular calcifications was assessed in
Wistar rats. Three groups were included, a control group, an InsP6
treated group (subjected to calcinosis induction by Vitamin D and
nicotine and treated with standard cream with a 2% of InsP6 as
potassium salt) and an InsP6 non-treated group (only subjected to
calcinosis induction). All rats were fed AIN 76-A diet (a purified
diet
in which InsP6 is undetectable). Animals were monitorized every 12
hours. After 60 hours of calcinosis treatment, all rats of the InsP6
non-treated group died and the rest were sacrificed. Aortas and hearts
were removed. A highly significant increase in the calcium content of
aorta and heart tissue was observed in the InsP6 non-treated rats (21
+/- 1 mg calcium / g dry aorta tissue, 10 +/- 1 mg calcium / g dry
heart tissue) when compared with controls (1.3 +/- 0.1 mg calcium / g
dry aorta tissue, 0.023 +/- 0.004 mg calcium / g heart dry tissue) and
InsP6 treated (0.9 +/- 0.2 mg calcium / g dry aorta tissue, 0.30 +/-
0.03 mg calcium / g dry heart tissue) animals. Only InsP6 non-treated
rats displayed important mineral deposits in aorta and heart. These
findings are consistent with the action of InsP6, as an inhibitor of
calcification of cardiovascular system.

PMID: 16146720
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Tom

Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh

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DEAD PEOPLE WALKING
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'Antioxidants May Increase the Risk of Developing Diabetes'

http://www.diabetesincontrol.com/index.php?option=com_content&view=ar...

The short version is that some pre-diabetic situations are avoided when
some level of prooxidants are present and the risk for diabetes
increased when they are lowered.  The prooxidants fight the diabetes in
a manner of speaking.

The supplementation of high doses would be considered to be actually
'pharmaceutical' and could theoretically be bad ..

Vitamin C is known to be an effective substance to free up iron in the
body so in theory it could be detrimental in  a situation of high
stored iron UNLESS during the same time one has a high iron BINDING
diet.

One would have to wonder exactly what the effects OF adding  vitamin C
to the mix of high iron in a human would be ..
WHEN one has 'not enough' iron binding substances in the body to
'handle' this freed up iron / abnormal amount of iron.

The effects of this phytic acid AND vitamin C would in theory be the
freeing up of stored iron and the phytic / phytate / chaff of your
grain would bind up this freed iron ..

Sooo you would have to have a combination of substances as what is
found NATURALLYin plant food to achieve the levels of antioxidants
they have shown .. for some reason .. that we NEED ..

Grain and legumes and fruit and vegetables which contain all the
nutrients and vitamins and such ..

Linus Pauling saw it .. oxidation / vitamin C needed .. but could
never figure out WHERE the need for vitamin C / antioxidants was
coming from .. the oxidation part of it.

He just knew it was oxidation ..

ALL plant foods contain this phytic acid in lesser to higher
amounts ..

ALL plant foods contain 'antioxidants' ..

Lower oxidation down to a 'normal' amount which we
seem to KNOW we NEED to do but not HOW to do ..

This free bisphosphonate found in your food when consumed ..
'coincidentally' ..
lowers oxidation in the fish and coincidentally binds up iron.

So you may think the scientists found the fish is being killed but the
scientists
think it is a good sign this lowering of 'markers'  which are
considered by them
to BE bad .. ?

But WHY would the fish DO better .. ?

One might wonder what they are naturally feeding these fish and not
taking into
account for minute portions of the diet .. ?
Such as they did when they feed mice fifty percent
of their diet meat / fish meal .. ?

They probably feed them pig guts or something without any
seaweed at .. all ..

----------

My free bisphosphonate .. again. http://tinyurl.com/2wuhuk

"Improved antioxidant status and lipid and protein peroxidation"

Lipid peroxidation, protein oxidant and antioxidant status
of muscle, intestine and hepatopancreas for juvenile Jian
carp (Cyprinus carpio var. Jian) fed graded levels of myo-inositol
Wei-Dan Jianga, b, Lin Fenga, b, Yang Liua, b, Jun Jianga, b,
Kai Hua, b, Shu-Hong Lia and Xiao-Qiu Zhoua, b, ,
aAnimal Nutrition Institute, Sichuan Agricultural University,
Sichuan, Ya’an, 625014, China
bKey Laboratory for Animal Disease-Resistance Nutrition of
China Ministry of Education, Sichuan Agricultural University,
Sichuan, Ya’an, 625014, China
Received 27 June 2009;  revised 14 August 2009;  accepted 21 October
2009.
Available online 29 October 2009.

Abstract
Lipid peroxidation, protein oxidant and antioxidant status of
muscle, intestine and hepatopancreas in juvenile Jian carp
(Cyprinus carpio var. Jian) fed graded levels of myo-inositol
(MI) (163.5, 232.7, 384.2, 535.8, 687.3, 838.8 and 990.3 mg/kg
diet) for 60 days were investigated.
Total tissue malondialdehyde (MDA) and protein carbonyl (PC)
content showed a downward trend to a point (P < 0.05).
Conversely, total tissue anti-hydroxyl radical (AHR), catalase
(CAT), glutathione-S-transferase (GST), glutathione peroxidase
(GPx), glutathione reducase (GR) activities and glutathione
(GSH) content were generally higher in MI-supplemented diets
than MI-unsupplemented diet (P < 0.05).
Muscle and intestinal superoxide dismutase (SOD), and
intestinal anti-superoxide anion (ASA) were increased by MI
supplementation (P < 0.05), whereas these parameters in the
other tissue showed no alterations (P > 0.05).
These results indicated that antioxidant status was improved,
and lipid peroxidation and protein oxidant were depressed in
muscle, intestine and hepatopancreas by MI.

Keywords: Jian carp (Cyprinus carpio var. Jian); Myo-inositol;
Lipid peroxidation; Protein oxidant; Hydroxyl radicals;
Antioxidant enzyme
doi:10.1016/j.foodchem.2009.10.062

Copyright © 2009 Published by Elsevier Ltd.

-----------

Phytate (Myo-inositol hexakisphosphate) inhibits cardiovascular
calcifications in rats.
Front Biosci. 2006 Jan 1;11:136-42.
Grases F, Sanchis P, Perello J, Isern B, Prieto RM, Fernandez-
Palomeque
C, Fiol M, Bonnin O, Torres JJ.
University Institute of Health Sciences Research (IUNICS), University
of Balearic Islands.

Calcification is an undesirable disorder, which frequently occurs in
the heart vessels. In general, the formation of calcific vascular
lesions involves complex physicochemical and molecular events.
Calcification (hydroxyapatite) is initiated by injury and is
progressed
by promoter factors and/or the deficit of inhibitory signals.
Myo-inositol hexakisphosphate (phytate, InsP6) is found in organs,
tissues and fluids of all mammals and exhibits an important capacity
as
a crystallization inhibitor of calcium salts in urine and soft
tissues.
The levels found clearly depend on the dietary intake but it can also
be absorbed topically. In this paper, the capacity of InsP6 as a
potential inhibitor of cardiovascular calcifications was assessed in
Wistar rats. Three groups were included, a control group, an InsP6
treated group (subjected to calcinosis induction by Vitamin D and
nicotine and treated with standard cream with a 2% of InsP6 as
potassium salt) and an InsP6 non-treated group (only subjected to
calcinosis induction). All rats were fed AIN 76-A diet (a purified
diet
in which InsP6 is undetectable). Animals were monitorized every 12
hours. After 60 hours of calcinosis treatment, all rats of the InsP6
non-treated group died and the rest were sacrificed. Aortas and
hearts
were removed. A highly significant increase in the calcium content of
aorta and heart tissue was observed in the InsP6 non-treated rats (21
+/- 1 mg calcium / g dry aorta tissue, 10 +/- 1 mg calcium / g dry
heart tissue) when compared with controls (1.3 +/- 0.1 mg calcium / g
dry aorta tissue, 0.023 +/- 0.004 mg calcium / g heart dry tissue)
and
InsP6 treated (0.9 +/- 0.2 mg calcium / g dry aorta tissue, 0.30 +/-
0.03 mg calcium / g dry heart tissue) animals. Only InsP6 non-treated
rats displayed important mineral deposits in aorta and heart. These
findings are consistent with the action of InsP6, as an inhibitor of
calcification of cardiovascular system.

PMID: 16146720
Who loves ya.
Tom

Jesus Was A Vegetarian!
http://tinyurl.com/2r2nkh

Man Is A Herbivore!
http://tinyurl.com/a3cc3

DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk